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A novel method with two variations is proposed with which the number of positive and negative zeros of a polynomial with real co-efficients and degree $n$ can be restricted with significantly better determinacy than that provided by the Descartes' rule of signs and also isolate quite successfully the zeros of the polynomial. The method relies on solving equations of degree smaller than that of the given polynomial. One can determine analytically the exact number of positive and negative zeros of a polynomial of degree up to and including five and also fully isolate the zeros of the polynomial analytically and with one of the variations of the method, one can analytically approach polynomials of degree up to and including nine by solving equations of degree no more than four. For polynomials of higher degree, either of the two variations of the method should be applied recursively. Classification of the roots of the cubic equation, together with their isolation intervals, is presented. Numerous examples are given.

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