Document Type

Theses, Ph.D

Rights

This item is available under a Creative Commons License for non-commercial use only

Disciplines

1.3 PHYSICAL SCIENCES

Publication Details

Successfully submitted for the award of Doctor of Philosophy (Ph.D), 2018.

Abstract

Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to a number of advantages in providing more physiologically relevant information and potentially more predictive data for in vivo tests. Extracellular matrix (ECM) proteins have been developed over in recent years to simulate a natural microenvironment for cells cultured in vitro. Conventional cell culture or 2D cell culture form monolayers of cells on a solid surface, which is typically polystyrene or glass, whereas a 3D culture system employ a porous growth matrix on which the cells grow. The transition from cell culture on a flat surface 2D to a 3D model in vitro is expected to mimic more realistic culture environments. However, the major limitation of 2D culture is their lack of structural architecture and stroma and not all types of normal epithelial cell are able to adhere and grow on 2D culture.

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