RNAi-based Glyconanoparticles Trigger Apoptotic Pathways for In Vitro and In Vivo Enhanced Cancer-Cell Killing

Authors

Joao Conde, Universidad de Zaragoza, Spain
Furong Tian, Technological University DublinFollow
Yulan Hernandez, Universidad de Zaragoza, Spain
Chenchen Bao, dInstitute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Translation Medicine, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, People’s Republic of China
Pedro Baptista, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, Portugal
Daxiang Cui, dInstitute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Translation Medicine, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, People’s Republic of China
Tobias Stoeger, bComprehensive Pneumology Centre, Institute of Lung Biology and Disease, Helmholtz Zentrum München, Neuherberg, Germany.
Jesus M. de la Fuentec, Universidad de Zaragoza, Spain

Document Type

Article

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Disciplines

Oncology

Publication Details

Nanoscale. 2015 May 21;7(19):9083-91.

Abstract

biotechnology and material science due to their amazing physical, chemical and biological properties. Here, siRNA GlycoNPs (AuNP@PEG@Glucose@siRNA) in comparison to PEGylated GlycoNPs (AuNP@PEG@Glucose) were applied in vitro to a luciferase-CMT/167 adenocarcinoma cancer cell line and in vivo via intratracheal instillation directly into the lung of B6 albino mice grafted with luciferase-CMT/167 adenocarcinoma cells. siRNA GlycoNPs but not PEGylated GlycoNPs induced the expression of pro-apoptotic proteins such as Fas/CD95 and caspases 3 and 9 in CMT/167 adenocarcinoma cells in a dose dependent manner, independent from the inflammatory response, evaluated by bronchoalveolar lavage cell counting. Moreover, in vivo pulmonary delivered siRNA GlycoNPs were capable of targeting c-Myc gene expression (a crucial regulator of cell proliferation and apoptosis) via in vivo RNAi in tumour tissue, leading to a ~80% reduction in tumour size without inflammation associated.

DOI

https://doi.org/10.1039/c4nr05742b.

Recommended Citation

Furlong, T. (et al) 2015, RNAi-based glyconanoparticles trigger apoptotic pathways for in vitro and in vivo enhanced cancer-cell killing. Nanoscale. 2015 May 21;7(19):9083-91. doi:10.1039/c4nr05742b.

Funder

FP7