Document Type

Article

Rights

This item is available under a Creative Commons License for non-commercial use only

Disciplines

Microbiology, Environmental biotechnology

Publication Details

Applied and environmental microbiology

Available here

Abstract

Atmospheric cold plasma (ACP) is a promising non-thermal technology effective against a wide range of pathogenic microorganisms. Reactive oxygen species (ROS) play a crucial inactivation role when air or other oxygen containing gases are used. With strong oxidative stress, cells can be damaged by lipid peroxidation, enzyme inactivation and DNA cleavage. Identifying ROS and understanding their role is important to advance ACP applications to a range of complex microbiological issues. In this study, the inactivation efficacy of in-package, high voltage (80 kVRMS) ACP (HVACP) and the role of intracellular ROS were investigated. Two mechanisms of inactivation were observed where reactive species were found to either react primarily with the cell envelope or to damage intracellular components. E. coli was inactivated mainly by cell leakage and low level DNA damage. Conversely, S. aureus was mainly inactivated by intracellular damage with significantly higher levels of intracellular ROS observed and little envelope damage. However, for both bacteria studied, increasing treatment time had a positive effect on intracellular ROS levels generated.

DOI

10.1128/AEM.02660-15

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