Document Type
Article
Rights
Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence
Disciplines
Environmental sciences, Freshwater biology, Nano-materials, Toxicology
Abstract
Polyamidoamine (PAMAM) dendrimers elicit systematically variable cyto- and eco-toxic responses, promising the basis for structure activity relationships governing nanotoxicological responses. Increased production of intracellular reactive oxygen species (ROS), genotoxicity and apoptosis due to in-vitro exposure of hepatocellular carcinoma cells to dendrimer generations G-4, G-5 and G-6 is demonstrated. A generation dependent increase in ROS and genotoxicity was observed, consistent with our previous studies. The responses correlate well with the number of surface amino groups per generation. Although ROS production initially increases approximately linearly, it saturates at higher doses. Notably, normalised to molar dose of surface amino groups, the dose-dependent ROS production for different generations overlap exactly, indicating that the response is due to these functional units. The percentage tail DNA formation is related to the generation and dose dependent production of intracellular ROS, at low levels. At the higher ROS levels, increased DNA damage is associated with the onset of necrosis.
DOI
https://doi.org/10.1016/j.aquatox.2013.01.020
Recommended Citation
Byrne, H., Naha, Pratap C.: Generation of Intracellular Reactive Oxygen Species and Genotoxicity Effect to Exposure of Nanosized Polyamidoamine (PAMAM) Dendrimers in PLHC-1 Cells in Vitro. Aquatic Toxicology, 132– 133, 61– 72 (2013). doi:10.1016/j.aquatox.2013.01.020
Funder
HEA
Included in
Other Pharmacology, Toxicology and Environmental Health Commons, Terrestrial and Aquatic Ecology Commons, Toxicology Commons
Publication Details
Aquatic Toxicology, 132– 133, 61– 72 (2013)