Title

Regeneration of Aged DMF for Use in Solid-Phase Peptide Synthesis

Authors

Jordan K. Magtaan, TU Dublin - Tallaght CampusFollow
Marc Devocelle, RCSIFollow
Fintan Kelleher, Institute of Technology, TallaghtFollow

Document Type

Article

Rights

This item is available under a Creative Commons License for non-commercial use only

Disciplines

1.4 CHEMICAL SCIENCES, Organic Chemistry

Publication Details

Journal of Peptide Science, 25 December 2018,

https://onlinelibrary.wiley.com/journal/10991387

Abstract

DMF, which is still the most commonly used solvent for Fmoc-SPPS, has the potential for degradation over time on exposure to air (and water vapour) and storage, to give dimethylamine and formic acid impurities. In particular, dimethylamine can lead to unwanted deprotection of the Fmoc group during for example the initial loading of Fmoc amino acids in SPPS, which leads reduced calculated loading values. We have found that treatment of such aged DMF by simple sparging with an inert gas (N₂), or vacuum sonication, can regenerate the DMF in order to restore loading levels back to those found for newer, fresh, DMF samples.

Recommended Citation

Magtaan, J. K., Devocelle, M., Kelleher, F. (2018) Regeneration of Aged DMF for Use in Solid-Phase Peptide Synthesis. Journal of Peptide Science, 25 December 2018,. doi:10.1002/psc.3139

DOI

10.1002/psc.3139