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2. ENGINEERING AND TECHNOLOGY
Changes in platelet function is a major complication in patients with cardiovascular disease. The authors have previously designed a portable, easy to use, low cost biochip for monitoring of anti-platelet therapy in cardiovascular disease patients . This method directly measures platelet function by measuring platelet adhesion to platelet-specific protein patterns using a simple, optical counting technique. The patterned surfaces are designed so that a single platelet adheres to a single protein spot and for imaging purposes, the proteins patterned on the surface are labelled with a fluorescent dye, as are the platelets that adhere to the patterned surface. Following image acquisition, the occupied (i.e. covered by a platelet) protein spots in each sample must be enumerated. The result, calculated as percent adhesion, constitutes a direct and straightforward measurement of platelet adhesion and, therefore, platelet function.
Lee, T., Gilmartin, N., Berry, D. & Courtney, J. (2019). Counting platelets: a novel image analysis algorithm for detecting and counting platelet adhesion on an assay pattern. IMVIP 2019: Irish Machine Vision & Image Processing, Technological University Dublin, Dublin, Ireland, August 28-30.# doi:10.21427/cgpb-qj33