Document Type

Article

Rights

This item is available under a Creative Commons License for non-commercial use only

Disciplines

Atomic, Molecular and Chemical Physics, Physical chemistry, Cell biology,, Pharmacology and pharmacy

Publication Details

“Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking”, Ewelina Szafraniec, Katarzyna Majzner, Zeineb Farhane, Hugh J. Byrne, Malgorzata Lukawska, Irena Oszczapowicz, Stefan Chlopicki, Malgorzata Baranska, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 169, 152-160 (2016)

doi:10.1016/j.saa.2016.06.035.

Abstract

A broad spectroscopic characterization, using ultraviolet-visible (UV-vis) and Fourier transform infrared absorption as well as Raman scattering, of two commonly used anthracyclines antibiotics (DOX) daunorubicin (DNR), their epimers (EDOX, EDNR) and ten selected analogs is presented. The paper serves as a comprehensive spectral library of UV-vis, IR and Raman spectra of anthracyclines in the solid state and in solution. The particular advantage of Raman spectroscopy for the measurement and analysis of individual antibiotics is demonstrated. Raman spectroscopy can be used to monitor the in vitro uptake and distribution of the drug in cells, using both 488 nm and 785 nm as source wavelengths, with submicrometer spatial resolution, although the cellular accumulation of the drug is different in each case. The high information content of Raman spectra allows studies of the drug-cell interactions, and so the method seems very suitable for monitoring drug uptake and mechanisms of interaction with cellular compartments at the subcellular level.

DOI

10.1016/j.saa.2016.06.035.

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