Document Type

Theses, Ph.D

Rights

This item is available under a Creative Commons License for non-commercial use only

Disciplines

Optics, 1.6 BIOLOGICAL SCIENCES, Biochemistry and molecular biology, Biophysics

Publication Details

Thesis submitted in fulfilment of the requirements for the degree of
MPhil in Biochemistry, July 2011.

Abstract

The aim of this thesis was to investigate the effect of the VEGF family of growth factors and
cytokines TNF- α and IL-1β on oBRB function. The human RPE line ARPE-19 was
employed as an in vitro model of the oBRB. Two-Dimensional gel electrophoresis was
employed to study the effect of growth factors on the RPE proteome. The barrier properties of
the oBRB were evaluated by expression of the TJ proteins occludin or Zo-1, or claudins-1, 2,
3 and 4, determined by western blotting analysis. Claudins 2, 3 and 4 were found to be
significantly up-regulated by TNF-α, while interleukin 1 β-treated RPE showed increased
expression of Claudins 1 and 3.