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Biochemistry and molecular biology
Among several lipase producing actinomycete strains screened, Amycolatopsis mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. Culture condition and nutrient source modification studies involving carbon sources, nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity of 1.37 ± 0.103 IU/ml of culture medium was obtained in 96 h at 28 °C and pH 7.5 using linseed oil and fructose as carbon sources and a combination of phytone peptone and yeast extract (5:1) as nitrogen sources. In optimal culture conditions the lipase activity was enhanced 12-fold with a 2-fold increase in lipase specific activity. The lipase showed maximum activity at 60 °C and pH 8.0. The enzyme was stable between pH 5.0–9.0 and temperatures up to 60 °C. Lipase activity was significantly enhanced by Fe3+ and strongly inhibited by Hg2+. Li+, Mg 2+ and PMSF significantly reduced lipase activity, whereas other metal ions had no significant effect at 0.01 M concentration. A. mediterranei DSM 43304 lipase exhibited remarkable stability in the presence of a wide range of organic solvents at 25% (v/v) concentration for 24h. These features render this novel lipase attractive for potential biotechnological applications in organic synthesis reactions.
Dheeman D.S., Frias J.M, Henehan, G.T.M. (2010) Influence of Cultivation Conditions on the Production of a Thermostable Extracellular Lipase from Amycolatopsis Mediterranei DSM 43304. Journal of Industrial Microbiology & Biotechnology, 37, (1),pp. 1-17