Correlation of Spectroscopic and Biochemical Assays Postionising Radiation Exposure in Human Skin Cell Analogues

Authors

Aidan Meade, Technological University DublinFollow
Hugh Byrne, Technological University DublinFollow
Fiona Lyng, Technological University DublinFollow

Document Type

Conference Paper

Rights

Available under a Creative Commons Attribution Non-Commercial Share Alike 4.0 International Licence

Publication Details

In Proceedings of OptoIreland 2005. SPIE vol.5826 (397), 2005.

Abstract

Raman spectroscopy, as an evaluation of the products of ionising radiation exposure in biological systems, has been utilised mainly in the evaluation of the impact of exposure in tissue, cellular constituents and live animals. It has also been recently demonstrated that Raman spectroscopy can demonstrate key spectroscopic changes in the live cell associated with significant apoptotic and necrotic chemical damage. The present preliminary work utilises Raman spectroscopy at 514.5 nm to evaluate the results of exposure to -rays in HaCaT cells from a Co-60 therapy source, in tandem with other biological assays. The results demonstrate that a number of spectral changes may be correlated with changes in the cell also identified in parallel biochemical assays.

DOI

https://doi.org/10.1117/12.611450

Recommended Citation

Meade, A., Byrne, H. & Lyng, F. (2005). Correlation of spectroscopic and biochemical assays postionising radiation exposure in human skin cell analogues. Proceedings of OptoIreland 2005. SPIE. vol. 5826, pg. 397. doi:10.1117/12.611450

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.