Document Type

Article

Rights

This item is available under a Creative Commons License for non-commercial use only

Publication Details

Published in Toxicology in Vitro 21 (2007) 438-448. Available online at www.sciencedirect.com

Abstract

This paper describes the in vitro cytotoxicity assessment of single walled carbon nanotubes (SWCNT) on A549 cells, a human lung cell line. Cellular viability was determined using the alamar blue (AB), neutral red (NR) and MTT assays, which evaluated metabolic, lyso-somal and mitochondrial activity respectively. In addition, the total protein content of the cells was measured using the coomassie bril-liant (CB) blue assay. Supernatants were also assayed for Adenylate Kinase (AK) release and Interleukin 8 (IL-8) which indicated a loss of cell membrane integrity and an in Xammation response respectively.

Included in

Physics Commons

Share

COinS