Functional and Pathological Analysis of Biological Systems using Vibrational Spectroscopy with Chemometric and Heuristic Approaches
Document Type Article
WHISPERS: First Workshop on Hyperspectral Imaging and Signal Processing: Evolution in Remote Sensing, IEEE Conference Proceedings, DOI: 10.1109/WHISPERS.2009.5288989, 2009.
Vibrational spectroscopy (Raman and FTIR microspectroscopy) is an attractive modality for the analysis of biological samples since it provides a complete non-invasive acquisition of the biochemical fingerprint of the sample. Studies in our laboratory have applied vibrational spectroscopy to the analysis of biological function in response to external agents (chemotherapeutic drugs, ionising radiation, nanoparticles), together with studies of the pathology of tissue (skin and cervix) in health and disease. Genetic algorithms have been used to optimize spectral treatments in tandem with the analysis of the data (using generalized regression neural networks (GRNN), artificial neural networks (ANN), partial least squares modelling (PLS), and support vector machines (SVM)), to optimize the complete analytical scheme and maximize the predictive capacity of the spectroscopic data. In addition we utilise variable selection techniques to focus on spectral features that provide maximal classification or regression of the spectroscopic data against analytical targets. This approach has yielded interesting insights into the variation of biochemical features of the biological system with its state, and has also provided the means to develop further the analytical and predictive capabilities of vibrational spectroscopy in biological analysis.